Can anyone tell me about Gas Chromatography?
chromatography means number of methods used separation of mixtures, in components of mixture introduced system of 2 phases, 1 of (the mobile phase) moving on or through other (stationary ) phase. differences in distribution of components between 2 phases cause them travel @ different speeds in system , separate. in gas chromatography (gc) stationary phase can nonvolatile liquid held on surface of finely divided solid. contained in metal or glass column held in oven , mobile phase (a gas) passed through column. mixture separated introduced vapour @ 1 end of column. component of mixture relatively soluble in stationary liquid travel more down column less soluble. @ end of column detector + associated recording equipment senses , displays results each component emerges.
gc powerful method separation of mixtures, not @ identifying separated components, although can combined other methods (eg mass spectrometry) @ identifying. gc restricted substances can vaporised without decomposition. 20% of chemical compounds suitable gc without form of sample modification, remainder nonvolatile or thermally unstable. many substances highly polar or ionisable functional groups not behave in gc. cases these examined using high performance liuid chromatography (hplc) restriction have able dissolve sample in solvent,
what advantages , disadvantages of gas chromatography?
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